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The laboratory diagnosis of fungal infection relies largely on direct as opposed to indirect methods. The use of malt and maltextracts for the propagation of yeasts and moulds is quite common. Reddish (1) described a culture medium prepared frommalt extract that was a satisfactory substitute for wort. Malt Extract Medium is similar to the formula of Galloway and Burgess(2) used for the detection, isolation and enumeration of yeasts and moulds.
Malt extract provides an acidic environment and nutrients favourable for growth and metabolism of yeasts and moulds.Mycological peptone rapidly gives a luxuriant growth with typical morphology and pigmentation. For mycological count, it isadvisable to adjust the reaction of medium more acidic with addition of 10% lactic acid. Antibiotics may be added as sterilesolutions to the molten medium immediately before pouring into sterile Petri plates (3) in order to suppress bacterial growth.
Type of specimen:
Clinical samples : skin scrappings
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions:
In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standardprecautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may bereferred in individual safety data sheets.
1. It is a general purpose medium which supports growth of bacterial and fungal cultures.
2. Further biochemical tests must be carried out for further identification.
Performance and Evaluation:
Performace of the medium is expected when used as per the direction on the label within the expiry period when stored atrecommended temperature.